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Catalog Number | orb547640 |
---|---|
Category | Antibodies |
Description | GCN2 EIF2AK4 Rabbit Monoclonal Antibody |
Species/Host | Rabbit |
Clonality | Monoclonal |
Clone Number | ACAA-5 |
Tested applications | FC, ICC, IF, IHC, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Immunogen | A synthesized peptide derived from human GCN2 Can phosphorylate the alpha subunit of EIF2 and may mediate translational control. |
Concentration | Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
UniProt ID | Q9P2K8 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Note | For research use only |
Application notes | WB 1:500-1:2000IHC 1:50-1:200ICC/IF 1:50-1:200FC 1:100 |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-GCN2 antibody.Counter stained with hematoxylin.
WB analysis of EIF2AK4 expression in HeLa Cell using anti-EIF2AK4 antibody.
Flow cytometric analysis of MCF-7 cells with GCN2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody.
ICC staining GCN2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
WB | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |
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