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Catalog Number | orb699488 |
---|---|
Category | Antibodies |
Description | GCLM, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | CPTC-GCLM-1 |
Tested applications | IHC-P, WB |
Reactivity | Human |
Isotype | Mouse IgG2a, kappa |
Immunogen | Recombinant human full-length GCLM protein was used as the immunogen for the GCLM antibody. |
Dilution range | Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This GCLM antibody is available for research use only. |
UniProt ID | P48507 |
Storage | Store the GCLM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the GCLM antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
SDS-PAGE analysis of purified, BSA-free GCLM antibody as confirmation of integrity and purity.
IHC staining of FFPE human tonsil with GCLM antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil with GCLM antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) A549, 2) A431 and 3) HepG2 cell lysate with GCLM antibody. Expected molecular weight ~31 kDa.
IHC-P | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC-P | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC-P | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC-P | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC-P | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
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