GCHFR Antibody / GFRP

• Catalog Number: orb1825429
• Category: Antibodies
• Description: GTP cyclohydrolase I feedback regulatory protein (GFRP) is encoded by the gene GCHFR. GFRP mediates feedback inhibition of GTP cyclohydrolase I activity by tetrahydrobiopterin. GFRP also acts as a mediator for the stimulatory effect of phenylalanine on enzyme activity. L-phenylalanine reverses this inhibition. Cross-linking experiments have shown that GFRP is usually expressed as a homodimer or pentamer.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: GCHFR/7732
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2, kappa
• Immunogen: A recombinant fragment of human GCHFR protein was used as the immunogen for the GCHFR antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This GCHFR antibody is available for research use only.
• UniProt ID: P30047
• Storage: Aliquot the GCHFR antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human liver tissue with GCHFR antibody (clone GCHFR/7732). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free GCHFR antibody (clone GCHFR/7732) as confirmation of integrity and purity.

Western blot testing of human SK-BR-3 cell lysate with GCHFR antibody (clone GCHFR/7732). Predicted molecular weight ~12 kDa.