GATA4 Antibody (C-term)

• Catalog Number: orb1936179
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB13444
• Tested applications: FC, WB
• Predicted Reactivity: Mouse, Other, Rat
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 44565 Da
• Target: This GATA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 298-328 amino acids from the C-terminal region of human GATA4.
• UniProt ID: P43694
• NCBI: NP_002043.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Transcription factor GATA-4, GATA-binding factor 4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of anti-GATA4 Antibody (C-term) in CEM cell line lysates (35 ug/lane). GATA4 (arrow) was detected using the purified Pab.

Western blot analysis of GATA4 (arrow) using rabbit polyclonal GATA4 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GATA4 gene (Lane 2).

GATA4 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.