GATA3 antibody

• Catalog Number: orb1151475
• Category: Antibodies
• Description: Rabbit monoclonal antibody to GATA3
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: GATA3/4550R
• Tested applications: IHC-P, WB
• Reactivity: Human, Rat
• Isotype: Rabbit IgG, kappa
• Immunogen: A conserved peptide between the GATA trans-activation and DNA-binding domain was used as the immunogen for the recombinant GATA3 antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant GATA3 antibody is available for research use only.
• UniProt ID: P23771
• Storage: Aliquot the recombinant GATA3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant GATA3 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human MCF7 cell lysate with recombinant GATA3 antibody (clone GATA3/4550R). Observed molecular weight: ~50 kDa (full length), ~37 kDa (splice form).

IHC staining of FFPE human bladder carcinoma tissue with recombinant GATA-3 antibody (clone GATA3/4550R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Negative control: IHC staining of FFPE human brain tissue using recombinant GATA3 antibody (clone GATA3/4550R) at 2 ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.