GAPDH Mouse Monoclonal Antibody (Loading Control)

• Catalog Number: orb500708
• Category: Antibodies
• Description: GAPDH Mouse Monoclonal Antibody (Loading Control)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 4F8
• Tested applications: WB
• Predicted Reactivity: Canine, Gallus, Monkey, Porcine, Rabbit, Sheep
• Reactivity: Hamster, Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinded Human GAPDH
• Concentration: 1mg/ml
• Dilution range: WB=1:5000-100000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 38 kDa
• Target: GAPDH
• UniProt ID: P04406
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: 38 kDa BFA-dependent ADP-ribosylation substrate; A
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Althenayyan, Saleh et al. Alternatively Spliced Isoforms of MUC4 and ADAM12 as Biomarkers for Colorectal Cancer Metastasis J Pers Med, 13, 135 (2023)

PubMed: 36675796

Sample: Lane 1: Human HEK293 cell lysates, Lane 2: Human Hela cell lysates, Lane 3: Human SH-SY5Y cell lysates, Lane 4: Mouse NIH/3T3 cell lysates, Lane 5: Hamster CHO cell lysates, Lane 6: Rat Brain tissue lysates, Primary: Anti-GAPDH (orb500708) at 1/200000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 38 kDa, Observed band size: 38 kDa.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GAPDH-Loading Control) Monoclonal Antibody, Unconjugated (orb500708) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GAPDH-Loading Control) Monoclonal Antibody, Unconjugated (orb500708) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human gastric), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GAPDH-Loading Control) Monoclonal Antibody, Unconjugated (orb500708) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human gastric), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GAPDH-Loading Control) Monoclonal Antibody, Unconjugated (orb500708) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GAPDH-Loading Control) Monoclonal Antibody, Unconjugated (orb500708) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GAPDH-Loading Control) Monoclonal Antibody, Unconjugated (orb500708) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GAPDH-Loading Control) Monoclonal Antibody, Unconjugated at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GAPDH-Loading Control) Monoclonal Antibody, Unconjugated at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GAPDH-Loading Control) Monoclonal Antibody, Unconjugated (orb500708) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GAPDH-Loading Control) Monoclonal Antibody, Unconjugated (orb500708) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GAPDH-Loading Control) Monoclonal Antibody, Unconjugated at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GAPDH-Loading Control) Monoclonal Antibody, Unconjugated at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat spleen), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GAPDH-Loading Control) Monoclonal Antibody, Unconjugated at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat spleen), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GAPDH-Loading Control) Monoclonal Antibody, Unconjugated at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

Sample: Lane 1: Rat Cerebrum tissue lysates, Lane 2: Rat Heart tissue lysates, Lane 3: Human A549 cell lysates, Lane 4: Human Jurkat cell lysates, Lane 5: Human Huvec cell lysates, Lane 6: Human Hela cell lysates, Lane 7: Human U2os cell lysates, Lane 8: Human HepG2 cell lysates, Primary: Anti-GAPDH (orb500708) at 1/50000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 38 kDa, Observed band size: 38 kDa.

Sample: Lane 1: Skin (Mouse) Lysate at 40 ug, Lane 2: Testis (Mouse) Lysate at 40 ug, Lane 3: Adrenal gland (Mouse) Lysate at 40 ug, Primary: Anti-GAPDH (orb500708) at 1/1 000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 38 kDa, Observed band size: 38 kDa.

Tissue/Cell: Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (GAPDH-Loading Control) monoclonal Antibody, Unconjugated (orb500708) 1:100, 90 minutes at 37°C, followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.