GAPDH antibody

• Catalog Number: orb555879
• Category: Antibodies
• Description: Rabbit polyclonal antibody to GAPDH
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC-P, IP, WB
• Reactivity: Bacteria, Bovine, Canine, Drosophila, E. coli, Fish, Gallus, Hamster, Human, Insect, Mammal, Monkey, Mouse, Other, Plant, Porcine, Rabbit, Rat, Yeast, Zebrafish
• Isotype: IgG
• Immunogen: Recombinant protein encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary.
• Concentration: 0.69 mg/ml
• Dilution range: WB: 1:5000-1:100000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000 , IP: 1:100-1:500
• Form/Appearance: Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
• Purity: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• MW: 36
• Target: GAPDH
• UniProt ID: P04406
• Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles.
• Buffer/Preservatives: 0.025% ProClin 300. 1XPBS, 1% rAlbumin, 20% Glycerol (pH7)
• Alternative names: glyceraldehyde-3-phosphate dehydrogenase , G3PD ,
• Note: For research use only
• Application notes: Positive control: PC-12 , Rat2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , E.coli M15
• Expiration Date: 12 months from date of receipt.

References

Hu Zheng 1, Liuyang Wu 1, Huisheng Yuan miR-30b-5p targeting GRIN2A inhibits hippocampal damage in epilepsy Open Med (Wars), 18, 20230675 (2023)

PubMed: 37016703

Applications

WB

Reactivity

Mouse

Immunofluorescence analysis of HeLa cells using GAPDH antibody

Immunohistochemical staining of human salivary gland cancer using GAPDH antibody

Immunohistochemical staining of mouse intestine using GAPDH antibody

Immunohistochemical staining of rat testis using GAPDH antibody

Immunoprecipitation analysis of 293T whole cell using GAPDH antibody

Western blot analysis of Various whole cell using GAPDH antibody

Western blot analysis of Various whole cell using GAPDH antibody

Western blot analysis of Whole cell extract using GAPDH antibody

Western blot analysis of Various whole cell using GAPDH antibody

Western blot analysis of PC-12 whole cell lysate(Lane 1), Rat2 whole cell lysate(Lane 2) using GAPDH antibody