GAPDH antibody

• Catalog Number: orb420355
• Category: Antibodies
• Description: GAPDH antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: GAPDH Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminus of human Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH).
• Concentration: 1.17 mg/ml
• Dilution range: ELISA: User Optimized, WB: 1:1000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-GAPDH Antibody is directed against human GAPDH protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest that this antibody would react with GAPDH from a wide range of organisms, including most vertebrates and some yeast. Broad reactivity makes this antibody an excellent loading control.
• Conjugation: Unconjugated
• UniProt ID: P04406
• NCBI: 7669492
• Storage: Store Anti-GAPDH antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-GAPDH antibody, rabbit anti-Glyceralde
• Note: For research use only
• Application notes: Anti-GAPDH Antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~36 kDa in size corresponding to GAPDH by western blotting in the appropriate cell lysate or extract. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines, making it ideal for use as a loading control marker. This Anti-GAPDH Antibody product is a new product replacing p/n 600-401-A33.
• Expiration Date: 12 months from date of receipt.

Western Blot of Rabbit anti-GAPDH antibody. Lane 1: Opal Pre-stained ladder. Lane 2: NIH/3T3 Lysate (p/n orb348714). Lane 3: HEK293 lysate (p/n orb348669). Lane 4: MOLT-4 Lysate (p/n orb348696). Lane 5: A549 Lysate (p/n orb348675). Lane 6: HeLa Lysate (p/n orb348668). Lane 7: NIH 3T3 Nuclear Lysate (orb348724). Lane 8: HeLa Nuclear Lysate (p/n orb348671). Lane 9: Jurkat Lysate (p/n orb348674). Lane 10: Opal Pre-stained ladder. Load: 35 µg per lane. Primary antibody: GAPDH antibody at 1:1000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n orb347654) at 1:70000 for 30 min at RT. Blocking Buffer: orb348637 for 30 min at RT. Predicted/Observed size: 36 kDa for GAPDH.