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Catalog Number | orb18774 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to GAPDH |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC, WB |
Reactivity | Canine, Human, Mouse, Porcine |
Dilution range | ELISA: 1:128000, WB: 0.001-0.003 μg/ml, IHC-P: 2.5 μg/ml, IF/ICC: 10μg/ml |
Conjugation | Unconjugated |
MW | 36.1 |
Target | GAPDH (C Terminus) Loading Control |
Entrez | 2597 |
Protein Sequence | HQVVSSDFNSDT |
RRID | AB_10750737 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti GAPDH antibody, anti glyceraldehyde-3-phospha Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx 35kDa band observed in lysates of Human Tonsil, Mouse Spleen, and cell lines HeLa, A431 and 293 (calculated MW of 36.1kDa according to NP_002037). Recommended concentration: 0.01-0.03 μg/ml. |
Expiration Date | 12 months from date of receipt. |
Hain, Anika et al. IL-2 Inducible Kinase ITK is Critical for HIV-1 Infection of Jurkat T-cells Sci Rep, 8, 3217 (2018)
Immunohistochemical staining of Human Pancreas using GAPDH antibody.
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells using GAPDH antibody.
Immunofluorescence analysis of paraformaldehyde fixed A549 cells using GAPDH antibody.
Western blot analysis of Human Liver (A), Tonsil (B) and (0.3ug/ml) Rat Brain (C) lysate using GAPDH antibody
Western blot analysis of HEK293 (A) and HeLa (B) cell lysate using GAPDH antibody
orb18774 (0.5 µg/mL) staining of HeLa (A), HEK293 (B), LNCaP (C) and A549 (D) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.
orb18774 (0.5 µg/mL) staining of Human Liver lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.
orb18774 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
ICC, IHC-P, IP, WB | |
Bacteria, Bovine, Canine, Drosophila, E. coli, Fish, Gallus, Hamster, Human, Insect, Mammal, Monkey, Mouse, Other, Plant, Porcine, Rabbit, Rat, Yeast, Zebrafish | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IHC-Fr, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IHC-P, WB | |
Canine, Gallus, Monkey, Rabbit, Sheep | |
Hamster, Human, Mouse, Porcine, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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