GAPDH Antibody

• Catalog Number: orb166925
• Category: Antibodies
• Description: Mouse monoclonal antibody to GAPDH.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1A10A10
• Tested applications: IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1
• Dilution range: WB: 1:2000-10000, IHC-P: 1:25, IF/ICC: 1:25
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 36053
• Target: GAPDH
• UniProt ID: P04406
• NCBI: NP_002037.2, NP_001243728.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Anti GAPDH antibody, anti GAPD antibody, anti Glyc
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-GAPDH Antibody at 1:1000 dilution + C6 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/15000 dilution.Observed band size: 36KDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-GAPDH Antibody at 1:1000 dilution. Lane 1: A431 whole cell lysates. Lane 2: C6 whole cell lysates. Lane 3: Hela whole cell lysates. Lane 4: HUVEC whole cell lysates.Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-GAPDH Antibody at 1:8000 dilution. Lane 1: Hela whole cell lysates. Lane 2: A549 whole cell lysates. Lane 3: COS-7 whole cell lysates. Lane 4: mouse brain lysates. Lane 5: C6 whole cell lysates. Lane 6: NIH/3T3 whole cell lysates. Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent image of Hela cells stained with XAF1 GAPDH Antibody. diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Immunohistochemical analysis of paraffin-embedded H.kidney section using GAPDH Antibody. diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H.stomach section using GAPDH Antibody. diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis of anti-GAPDH Monoclonal Antibody in CEM cell line lysates (35 μg/lane). GAPDH (arrow) was detected using the purified Mab.