GAPDH Antibody (C-term R248)

• Catalog Number: orb38656
• Category: Antibodies
• Description: Purified rabbit polyclonal antibody.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB16543
• Tested applications: FC, IF, IHC-P, WB
• Predicted Reactivity: Mouse, Porcine, Rat
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: Synthetic Peptide
• Dilution range: WB: 1:1000, IHC-P: 1:50-100, FACS: 1:10-50, IF/ICC: 1:10-50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 36053
• Target: GAPDH
• UniProt ID: P04406
• NCBI: NP_002037.2, NP_001243728.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Anti-Glyceraldehyde-3-phosphate dehydrogenase anti
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Xiaolan Huang 1, Xiangmin Luo 2, Suzhen Huang 2, Xiaoqing Chen 3, Lingling Qiu Inhibition of FoxO1 alleviates polycystic ovarian syndrome by reducing inflammation and the immune response Funct Integr Genomics, (2024)

PubMed: 38189995

Applications

WB

Reactivity

Rat

Western blot analysis of GAPDH Antibody (C-term R248) in A2058, A375, CEM cell line lysates (35 ug/lane). GAPDH (arrow) was detected using the purified Pab.

Confocal immunofluorescent analysis of GAPDH Antibody (C-term R248) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Flow cytometric analysis of HepG2 cells using GAPDH Antibody (C-term R248) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of lysates from Hela, HUVEC cell line (from left to right), using GAPDH Antibody (C-term R248). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.

Confocal immunofluorescent analysis of GAPDH Antibody (C-term R248) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human prostate carcinoma with GAPDH Antibody (C-term R248), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-GAPDH Antibody (C-term R248) at 1:1000 dilution. Lane 1: A431 whole cell lysate. Lane 2: Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.