Gap43 Antibody

• Catalog Number: orb1273526
• Category: Antibodies
• Description: Gap43 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse, Rat
• Immunogen: GAP-43 (Ser41) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser41 of rat GAP-43.
• Concentration: batch dependent
• Dilution range: In some tissues the antibody also recognizes a higher molecular weight band that is also recognized by the Pan GAP-43 antibody and this band may be a GAP-43 aggregate or oligomer. Immunolabeling of the GAP-43 protein in Western blots of rat brain is blocked by the Ser41 phosphopeptide used as antigen but not by the corresponding non-phosphopeptide. Note: the GAP-43 protein migrates on SDS-PAGE as a 50 kDa band. The antibody is purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns. Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Initial recommended range of dilutions: 1:500 to 1:2000. Applications include Dot blots (DB) and Western blots (WB). Immunohistochemistry (IHC) application has not yet been determined. Human, mouse and rat have 100% amino acid sequence identity with for the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 50
• Target: Gap43
• UniProt ID: P07936
• NCBI: P07936
• Storage: For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20°C.
• Note: For research use only
• Application notes: In some tissues the antibody also recognizes a higher molecular weight band that is also recognized by the Pan GAP-43 antibody and this band may be a GAP-43 aggregate or oligomer. Immunolabeling of the GAP-43 protein in Western blots of rat brain is blocked by the Ser41 phosphopeptide used as antigen but not by the corresponding non-phosphopeptide. Note: the GAP-43 protein migrates on SDS-PAGE as a 50 kDa band. The antibody is purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns. Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Initial recommended range of dilutions: 1:500 to 1:2000. Applications include Dot blots (DB) and Western blots (WB). Immunohistochemistry (IHC) application has not yet been determined. Human, mouse and rat have 100% amino acid sequence identity with for the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
• Expiration Date: 12 months from date of receipt.

Western blot of rat cortex lysate showing phospho-specific immunolabeling of the ~50k GAP-43 protein phosphorylated at Ser41.