Gamma Catenin Antibody / Plakoglobin

• Catalog Number: orb385949
• Category: Antibodies
• Description: Plakoglobin, or gamma Catenin, is a common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of Plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton (By similarity). [UniProt]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 15F11
• Tested applications: FACS, IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant full length chicken gamma Catenin was used as the immunogen for the Plakoglobin antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Plakoglobin antibody is available for research use only.
• UniProt ID: P14923
• Storage: Store the Plakoglobin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The stated application concentrations are suggested starting amounts. Titration of the Plakoglobin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of human HeLa cells with Plakoglobin antibody (clone 15F11, green) and Reddot nuclear stain (red).

Western blot testing of human 1) HT20, 2) HEK293, 3) A431, 4) MCF7, 5) HEPG2 and 6) A549 cell lysate with Plakoglobin antibody (clone 15F11). Predicted molecular weight: 80-87 kDa.

Flow cytometry testing of PFA-fixed human MCF7 cells with Plakoglobin antibody (clone 15F11); Red=isotype control, Blue=Plakoglobin antibody.

Western blot testing of human 1) HeLa and 2) SK-BR-3 cell lysate with Plakoglobin antibody (clone 15F11). Predicted molecular weight: 80-87 kDa.

IHC staining of FFPE human cervix with Plakoglobin antibody (clone 15F11). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.