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Catalog Number | orb196251 |
---|---|
Category | Antibodies |
Description | Galectin 3/LGALS3 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IF, IHC, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human Galectin 3 recombinant protein (Position: K139-I250). Human Galectin 3 shares 88% and 84% amino acid (aa) sequences identity with mouse and rat Galectin 3, respectively. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 26152 MW |
UniProt ID | P17931 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Galectin-3;Gal-3;35 kDa lectin;Carbohydrate-bindin Read more... |
Note | For research use only |
Application notes | WB: The detection limit for Galectin 3 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of U20S cells using anti-Galectin-3 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of Galectin-3 using anti-Galectin-3 antibody.Lane 1:human HeLa cell; 2:human SiHa cell; 3:human U87 cell; 4:human Hacat cell; 5:human PC-3 cell; 6:human T47D cell.
IF analysis of Galectin-3 using anti-Galectin-3 antibody.Galectin-3 was detected in immunocytochemical section of U20S cell.
IF analysis of Galectin-3 using anti-Galectin-3 antibody. Galectin-3 was detected in an immunocytochemical section of SiHa cells.
IHC analysis of Galectin-3 using anti-Galectin-3 antibody.Galectin-3 was detected in paraffin-embedded section of Human Lung Cancer Tissue.
IHC analysis of Galectin-3 using anti-Galectin-3 antibody.Galectin-3 was detected in paraffin-embedded section of Human Placenta Tissue.
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, ICC, IF, WB | |
Human, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
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