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Catalog Number | orb527053 |
---|---|
Category | Antibodies |
Description | Galectin 3/LGALS3 Antibody (monoclonal, 12B12) |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 12B12 |
Tested applications | FC, ICC, IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Mouse IgG2a |
Immunogen | E.coli-derived human Galectin 3 recombinant protein (Position: K139-I250). Human Galectin 3 shares 88% and 84% amino acid (aa) sequence identity with mouse and rat Galectin 3, respectively. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 28 kDa |
UniProt ID | P17931 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Galectin-3; Gal-3; 35 kDa lectin; Carbohydrate-bin Read more... |
Note | For research use only |
Application notes | Add 0.2ml of distilled water will yield a concentration of 500μg/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of U20S cells using anti-Galectin 3/LGALS3 antibody (Blue line).Isotype control antibody (Green line) was mouse IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis using anti-Galectin 3/LGALS3 antibody.Lane 1:HeLa cell;2:U87 cell;3:PC-3 cell.4:human T47D cell;5:human CACO-2 cell;6:human placenta tissue;7:human A549 cell;8:human HL-60 cell;9:rat lung tissue;10:rat testis tissue;11:mouse lung tissue.
IF analysis of Galectin 3/LGALS3 using anti-Galectin 3/LGALS3 antibody. Galectin 3/LGALS3 was detected in immunocytochemical section of A431 cells.
IF analysis of Galectin 3/LGALS3 using anti-Galectin 3/LGALS3 antibody. Galectin 3/LGALS3 was detected in immunocytochemical section of MCF-7 cells.
IHC analysis of Galectin 3/LGALS3 using anti Galectin 3/LGALS3 antibody. Galectin 3/LGALS3 was detected in paraffin-embedded section of human intestinal cancer tissue.
IHC analysis of Galectin 3/LGALS3 using anti Galectin 3/LGALS3 antibody. Galectin 3/LGALS3 was detected in paraffin-embedded section of human melanoma tissue.
IHC analysis of Galectin 3/LGALS3 using anti Galectin 3/LGALS3 antibody. Galectin 3/LGALS3 was detected in paraffin-embedded section of mouse small intestine tissue.
IHC analysis of Galectin 3/LGALS3 using anti Galectin 3/LGALS3 antibody. Galectin 3/LGALS3 was detected in paraffin-embedded section of rat small intestine tissue.
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