GAD67 Antibody

• Catalog Number: orb606596
• Category: Antibodies
• Description: This mAb recognizes a protein of 67kDa, which is identified as glutamic acid decarboxylase 1 (GDA1). There are two forms of glutamic acid decarboxylases (GADs) that are found in the brain: GAD65 (also known as GAD2) and GAD67 (also known as GAD1). GAD65 and GAD67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate-limiting step in the production of GABA (gamma-aminobutyric acid) from L-glutamic acid. Although both GAD s are found in the brain, GAD65 localizes to synaptic vesicle membranes in nerve terminals, while GAD67 is distributed throughout the cell. GAD67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD65 will transiently activate to assist in GABA production. The loss of GAD65 is detrimental and can impair GABA neurotransmission, however the loss of GAD67 is lethal.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: GAD1/2391
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A portion of amino acids 72-135 from the human protein were used as the immunogen for this GAD67 antibody.
• Dilution range: ELISA (order BSA/sodium azide-free format for coating),Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This GAD67 antibody is available for research use only.
• UniProt ID: Q99259
• Storage: Store the GAD67 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the GAD67 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human brain lysate with GAD67 antibody. Expected molecular weight ~67 kDa.

IHC testing of FFPE human brain with GAD67 antibody (clone GAD1/2391). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using GAD67 antibody (clone GAD1/2391). These results demonstrate the foremost specificity of the GAD1/2391 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free GAD67 antibody (clone GAD1/2391) as confirmation of integrity and purity.