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Catalog Number | orb1946668 |
---|---|
Category | Assays and Kits |
Description | gABA(Gamma-Aminobutyric Acid) ELISA Kit |
Range | 31.25-2000 pg/mL |
Target | gABA |
Sensitivity | 9.27 pg/mL |
Alternative names | 4-Aminobutyric Acid Read more... |
Note | For research use only |
Application notes | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Gamma-Aminobutyric Acid(gABA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Gamma-Aminobutyric Acid(gABA). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Gamma-Aminobutyric Acid(gABA) in the samples is then determined by comparing the OD of the samples to the standard curve. |
Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Expiration Date | Please enquire. |
Zebrafish | |
31.25-2000 pg/mL | |
16.7 pg/mL |
Human | |
31.25-2000 pg/mL | |
14.7 pg/mL |