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Catalog Number | orb1227826 |
---|---|
Category | Proteins |
Description | G-CSF Recombinant Protein |
Tested applications | ELISA, WB |
Tag | Tag Free |
Form/Appearance | Lyophilized |
Purity | > 95% as determined by SDS-PAGE.Endotoxin level is less than 1.0 EU per ug by the LAL method. |
MW | 18.7 kDa |
Target | G-CSF |
UniProt ID | P09919 |
Protein Sequence | Thr 31 - Pro 204 |
Source | HEK293 cells |
Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human G-CSF, Tag Free at 2 ug/mL (100 uL/well) can bind Biotinylated Human G-CSF R, His Tag with a linear range of 0.156-1.25 ug/mL. |
NCBI | NP_757373.1 |
Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | PBS, pH 7.4 |
Alternative names | G-CSF,CSF3,C17orf33,CSF3OS,MGC45931 Read more... |
Note | For research use only |
Application notes | This recombinant protein can be used for E, WB. For research use only. |
Expiration Date | 6 months from date of receipt. |
Human G-CSF, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
> 98% as determined by SDS-PAGE and HPLC. | |
18.7 kDa | |
E.Coli |
> 98% as determined by SDSPAGE and HPLC. | |
18.9 kDa | |
E.Coli |
Greater than 95% as determined by reducing SDS-PAGE. | |
18.8 KDa | |
Mammalian |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 44.8 kDa after removal of the signal peptide. The apparent molecular mass of G-CSF-hFc is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
Greater than 90% as determined by SDS-PAGE. | |
69.8 kDa | |
Mammalian cell |