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Catalog Number | orb1230574 |
---|---|
Category | Proteins |
Description | Furin Recombinant Protein |
Form/Appearance | Lyophilized |
Purity | Greater than 95% by SDS-PAGE gel and HPLC analyses. |
Target | FURIN |
UniProt ID | P09958 |
Protein Sequence | DLNVKAAWAQ GYTGHGIVVS ILDDGIEKNH PDLAGNYDPG ASFDVNDQDP DPQPRYTQMN DNRHGTRCAG EVAAVANNGV CGVGVAYNAR IGGVRMLDGE VTDAVEARSL GLNPNHIHIY SASWGPEDDG KTVDGPARLA EEAFFRGVSQ GRGGLGSIFV WASGNGGREH DSCNCDGYTN SIYTLSISSA TQFGNVPWYS EACSSTLATT YSSGNQNEKQ IVTTDLRQKC TESHTGTSAS APLAAGIIAL TLEANKNLTW RDMQHLVVQT SKPAHLNAND WATNGVGRKV SHSYGYGLLD AGAMVALAQN WTTVAPQRKC IIDILTEPKD IGKRLEVRKT VTACLGEPNH ITRLEHAQAR LTLSYNRRGD LAIHLVSPMG TRSTLLAARP HDYSADGFND WAFMTTHSWD EDPSGEWVLE IENTSEANNY GTLTKFTLVL YGTAPEGLPV PPESSGCKTL TSSQACVVCE EGFSLHQKSC VQHCPPGFAP QVLDTHYSTE NDVETIRASV CAPC |
Source | High-5 Insect cells |
Biological Activity | Biological activity was measured by its ability to cleave the fluorogenic peptide substrate Boc-Arg-Val-Arg-Arg-AMC (Bachem Catalog# I-1645.0025). Endotoxin level is less than 0.1 ng per μg (1EU/μg). |
NCBI | NP_001276752.1 |
Storage | The lyophilized Furin recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted Furin stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing Furin recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
Alternative names | FUR, PACE, SPC1, PCSK3, FUR, Furin, Dibasic-proces Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 61.0 kDa after removal of the signal peptide. The apparent molecular mass of FURIN-His is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
Unconjugated | |
95% | |
136.3 kDa | |
SARS S protein (R667A, K968P, V969P), His Tag (orb750333) is expressed from human 293 cells (HEK293). It contains AA Ser 14 - Pro 1195 (Accession # AAP13567.1 (R667A, K968P, V969P)). The recombinant protein is expressed with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (K968P, V969P) and alanine substitutions (R667A) are introduced to stabilize the trimeric prefusion state of SARS-CoV S protein and abolish the furin cleavage site, respectively. |
ELISA, WB | |
Greater than 95% by SDS-PAGE gel analyses | |
48.8 KDa | |
E.Coli |
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