FTL Antibody / Ferritin Light Chain

• Catalog Number: orb699626
• Category: Antibodies
• Description: Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: FTL/3872R
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: Recombinant full-length human FTL protein was used as the immunogen for the recombinant Ferritin Light Chain antibody.
• Dilution range: Western blot: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant Ferritin Light Chain antibody is available for research use only.
• UniProt ID: P02792
• Storage: Store the recombinant Ferritin Light Chain antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant Ferritin Light Chain antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human kidney lysate with recombinant Ferritin Light Chain antibody (clone FTL/3872R). Predicted molecular weight: ~20 kDa.

IHC staining of FFPE human breast carcinoma with recombinant Ferritin Light Chain antibody (clone FTL/3872R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant Ferritin Light Chain antibody as confirmation of integrity and purity.