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Catalog Number | orb639670 |
---|---|
Category | Antibodies |
Description | Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts. |
Species/Host | Mouse |
Clonality | Recombinant |
Clone Number | rFTL/1388 |
Tested applications | IHC-P, WB |
Reactivity | Human |
Isotype | Mouse IgG1, kappa |
Immunogen | A human recombinant partial protein (amino acids 38-165) was used as the immunogen for the recombinant FTL antibody. |
Dilution range | Immunohistochemistry (FFPE): 0.1-0.2ug/ml,Western blot: 1-2ug/ml |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This recombinant FTL antibody is available for research use only. |
UniProt ID | P02792 |
Storage | Store the recombinant FTL antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
IHC staining of FFPE human pancreas with recombinant FTL antibody (clone rFTL/1388). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC staining of FFPE human pancreas with recombinant FTL antibody (clone rFTL/1388). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Western blot testing of human kidney lysate with recombinant FTL antibody (clone rFTL/1388). Predicted molecular weight: ~20 kDa.
SDS-PAGE analysis of purified, BSA-free recombinant FTL antibody (clone rFTL/1388) as confirmation of integrity and purity.
ELISA, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC-P, WB | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
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