FTL Antibody / Ferritin light chain

• Catalog Number: orb606893
• Category: Antibodies
• Description: Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: FTL/1387
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: Amino acids 38-165 from the human protein were used as the immunogen for this FTL antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.1-0.2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This FTL antibody is available for research use only.
• UniProt ID: P02792
• Storage: Store the FTL antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the FTL antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human prostate with FTL antibody. Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min and allow to cool before testing.

Western blot testing of human HeLa cell lysate with FTL antibody. Predicted molecular weight ~20 kDa.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using FTL antibody. These results demonstrate the foremost specificity of the FTL/1387 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free FTL antibody as confirmation of integrity and purity.