FTH1 Antibody / Ferritin heavy chain

• Catalog Number: orb639668
• Category: Antibodies
• Description: Mammalian ferritins consist of 24 subunits made up of 2 types of poly-peptide chains, ferritin heavy chain and ferritin light chain, which each have unique functions. Ferritin heavy chains catalyze the first step in iron storage, the oxidationof FeI(II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe(III). The most prominent role of mammalian ferritins is to provide iron-buffering capacity to cells. In addition to iron buffering, heavy chain ferritin is also involved in the regulation of thymidine biosynthesis via increased expression of cytoplasmic serine hydroxymethyltransferase, which is a limiting factor in thymidylate synthesis in MCF-7 cells. Light chain ferritin is involved in cataracts by at least two mechanisms: hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed; and oxidative stress, an important factor in the development of aging-related cataracts.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: FTH/2081
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2c, kappa
• Immunogen: A human recombinant partial protein (amino acids 58-180) were used as the immunogen for the FTH1 antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.1-0.2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This FTH1 antibody is available for research use only.
• UniProt ID: P02794
• Storage: Store the FTH1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human pancreas with FTH1 antibody (clone FTH/2081). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE human pancreas with FTH1 antibody (clone FTH/2081). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Western blot testing of human HeLa cell lysate with FTH1 antibody (clone FTH/2081). Predicted molecular weight ~21 kDa.

SDS-PAGE analysis of purified, BSA-free FTH1 antibody (clone FTH/2081) as confirmation of integrity and purity.