FTaseW102T_Y154TGST protein

• Catalog Number: orb533684
• Category: Proteins
• Description: FTase is one of the three mammalian protein prenyltransferases. The double mutant of FTase was engineered to efficiently transfer biotin-geranylpyrophosphate (BGPP, Cat.# orb64043) onto its native protein substrates. This approach allows the detection of femtomolar amounts of prenylatable proteins in eukaryotic cells and tissues as well as effective inhibitor screening.
• Clonality: Recombinant
• Form/Appearance: liquid (Supplied in 25 mM HEPES pH 7.2, 40 mM NaCl and 2 mM DTT)
• Purity: > 90% (SDS-PAGE)
• MW: Theoretical MW: GST-α: 73 kDa, β: 48 kDa
• Source: E. coli
• Storage: store at -80 °C
• Note: For research use only
• Application notes: Activity: 1 pmol of FTase double mutant will transfer 0.4 pmol of BGPP to H-Ras in 15 min at 37°C.
• Expiration Date: 6 months from date of receipt.