fT3(Free Triiodothyronine) ELISA Kit

• Catalog Number: orb1806506
• Category: Assays and Kits
• Description: fT3(Free Triiodothyronine) ELISA Kit
• Reactivity: All
• Range: 937.5-60000 fg/mL
• Target: fT3
• Sensitivity: 258.3 fg/mL
• Alternative names: FT3
• Note: For research use only
• Application notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Free Triiodothyronine(fT3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Free Triiodothyronine(fT3). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Free Triiodothyronine(fT3) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Sample Types: Serum, plasma and other biological fluids
• Expiration Date: Please enquire.