Fractalkine antibody

• Catalog Number: orb345153
• Category: Antibodies
• Description: Fractalkine antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Anti-Fractalkine antibody was prepared from rabbit antiserum after repeated immunizations with a truncated form of recombinant human Fractalkine protein from E. coli.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:20,000 - 1:50,000, WB: 1:1000-1:2000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Fractalkine is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human Fractalkine protein. A BLAST analysis was used to suggest cross-reactivity with Fractalkine from human sources based on 100% homology with the immunizing sequence. Cross-reactivity with Fractalkine from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: P78423
• NCBI: NP_002987.1
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Fractalkine Antibody, CX3CL1, Fractalk
• Note: For research use only
• Application notes: Fractalkine purified antibody has been tested in western blotting and suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~42 kDa in size corresponding to the human Fractalkine protein by western blotting in appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western Blot of Rabbit anti-Fractalkine antibody. Lane 1: Human Fractalkine Recombinant Protein. Lane 2: None. Load: 50 ng per lane. Primary antibody: Fractalkine antibody at 1:1000 for overnight at 4°C. Secondary antibody: DyLight™ 649 rabbit secondary antibody at 1:20000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 9 kDa, 9 kDa for Human Fractalkine. Other band(s): None.