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Fractalkine Antibody Biotin Conjugated
Catalog Number: orb345189
| Catalog Number | orb345189 |
|---|---|
| Category | Antibodies |
| Description | Fractalkine antibody (Biotin) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ELISA, WB |
| Reactivity | Human |
| Isotype | IgG |
| Immunogen | Anti-Fractalkine antibody was prepared from rabbit antiserum after repeated immunizations with a truncated form of recombinant human Fractalkine protein from E. coli. |
| Antibody Type | Primary Antibody |
| Concentration | 1.0 mg/mL |
| Dilution range | ELISA: 1:20,000 - 1:50,000, WB: 1:1000-1:2000 |
| Form/Appearance | Lyophilized |
| Purity | Anti-Fractalkine is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human Fractalkine protein. A BLAST analysis was used to suggest cross-reactivity with Fractalkine from human sources based on 100% homology with the immunizing sequence. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Rabbit Serum. Cross-reactivity with Fractalkine from other sources has not been determined. |
| Conjugation | Biotin |
| UniProt ID | P78423 |
| NCBI | NP_002987.1 |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Alternative names | rabbit anti-Fractalkine Biotin conjugated Antibody Read more... |
| Note | For research use only |
| Application notes | Fractalkine purified antibody has been tested in western blotting and suitable in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~42 kDa in size corresponding to the human Fractalkine protein by western blotting in appropriate cell lysate or extract. |
| Expiration Date | 12 months from date of receipt. |
Western blot of Biotin conjugated Rabbit Anti-Fractalkine primary antibody. Lane 1: Human Fractalkine. Lane 2: None. Load: 50 ng per lane. Primary antibody: Fractalkine Biotin conjugated antibody at 1:1000 for 60 min at RT. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40000 for 30 min at RT. Blocking: orb348637 for 30 min at RT. Observed/Predicted size: 9 kDa, 9 kDa for Human Fractalkine. Other band(s): None.
