FOXP4 Antibody

• Catalog Number: orb1826329
• Category: Antibodies
• Description: The FOXP4 (Scm-like with four MBT domains 2) gene shares high similarity with the Drosophila Scm (sex comb on midleg) gene. Sfmbt2 is a Polycomb group (PcG) gene that maps to the proximal region of Chromosome 2, and is a putative imprinted gene. Sfmbt2 is the first imprinted gene within this region to be identified. Studies indicate that six different translocations involving proximal chromosome 2 results in lethality when present as a maternal uniparental duplication.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-FOXP4-1G7
• Tested applications: FACS, IF
• Reactivity: Human
• Isotype: Mouse IgG2c
• Immunogen: A recombinant partial protein (within amino acids 302-541) from the human protein was used as the immunogen for the FOXP4 antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This FOXP4 antibody is available for research use only.
• UniProt ID: Q8IVH2
• Storage: Aliquot the FOXP4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of PFA-fixed human HeLa cells with FOXP4 antibody (clone PCRP-FOXP4-1G7) followed by goat anti-mouse IgG-CF488 (green). Staining observed in the cytoplasm and nucleus but not nucleoli.

SDS-PAGE analysis of purified, BSA-free FOXP4 antibody (clone PCRP-FOXP4-1G7) as confirmation of integrity and purity.

Flow cytometry testing of PFA-fixed human HeLa cells with FOXP4 antibody (clone PCRP-FOXP4-1G7) followed by goat anti-mouse IgG-CF488 (blue); Red = unstained cells.

Analysis of a HuProt (TM) microarray containing more than 19, 000 full-length human proteins using FOXP4 antibody (clone PCRP-FOXP4-1G7). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.