FOXP3 antibody

• Catalog Number: orb18370
• Category: Antibodies
• Description: Goat polyclonal antibody to FOXP3
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Reactivity: Human
• Dilution range: ELISA: 1:8000, WB: 1-3 μg/ml, IHC-P: 10ug/ml
• Conjugation: Unconjugated
• MW: 47.2; 43.4
• Target: FOXP3 / SCURFIN
• Entrez: 50943
• Protein Sequence: SQRPSRCSNPTPGP
• RRID: AB_10749065
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti FOXP3 antibody, anti SCURFIN antibody, anti f
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Tonsil, stains nuclei of some cells in the interfollicular area. Recommended concentration, 0.3-1 ug/ml.
• Expiration Date: 12 months from date of receipt.

orb18370 (1µg/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. A) Nuclear staining of scattered cells in the interfollicular area. B) High magnification of positive cells. This data was

orb18370 staining of CD25-sorted (Treg) human blood cells gathered by cytospin and detected by FITC (A) and in phase contrast (B).

Western blot analysis of Human Muscle(lane 1), MOLT4(lane 2) and Pancreas(lane 3) lysate using FOXP3 antibody

Flow cytometric analysis of Jurkat cells using FOXP3 antibody

Immunofluorescence analysis of Human blood cells using FOXP3 antibody