FoxO4 (phospho Ser197) rabbit pAb

SKU: orb764191

Description

Rabbit polyclonal antibody to FOXO4 (phospho-Ser197)

Images & Validation

• Tested Applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
• Reactivity: Human, Mouse

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human AFX around the phosphorylation site of Ser197. AA range:164-213
• Molecular Weight: 70
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-FOXO4 antibody, anti-AFX antibody, anti-AFX1 antibody, anti-MLLT7 antibody, anti-Forkhead box protein O4 antibody, anti-Fork head domain transcription factor AFX1 antibody

Western Blot analysis of various cells using Phospho-FoxO4 (S197) Polyclonal Antibody.

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using AFX (Phospho-Ser197) Antibody.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using AFX (Phospho-Ser197) Antibody. The picture on the right is blocked with the phospho peptide.