FOXO3 Antibody (N-term)

• Catalog Number: orb1936776
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB33291
• Tested applications: IHC-P, WB
• Predicted Reactivity: Mouse
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 71277 Da
• Target: This FOXO3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human FOXO3.
• UniProt ID: O43524
• NCBI: NP_001446.1, NP_963853.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Forkhead box protein O3, AF6q21 protein, Forkhead
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-FOXO3 Antibody (N-term) at 1:1000 dilution. Lane 1: MCF-7 whole cell lysate. Lane 2: SH-SY5Y whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 71 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

FOXO3 Antibody (N-term) western blot analysis in Hela, MCF-7 cell line lysates (35 ug/lane). This demonstrates the FOXO3 antibody detected the FOXO3 protein (arrow).

FOXO3 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of FOXO3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.