FOXO1/3 (pS322+S325/pS318+S321) antibody

• Catalog Number: orb665477
• Category: Antibodies
• Description: Rabbit polyclonal antibody to FOXO1/3 (Phospho-S322+S325/pS318+S321)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Porcine, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S322+S325/pS318+S321 of human FOXO1/3 protein. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1000, IHC-P: 1-50-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: FOXO1; FOXO3
• Entrez: 56484, 2308, 84482, 56458
• UniProt ID: G3V7R4, Q9R1E0, Q9WVH4, O43524, Q12778
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Anti-FOXO1 antibody, anti-FKHR antibody, anti-FOXO
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of FOXO1/3 (Phospho-S322+S325/pS318+S321) expression in Beas2B (A), A549 (B), HepG2 (C) whole cell lysates. (Predicted band size: 69; 71 kD; Observed band size: 72; 90 kD)

Immunohistochemical analysis of FOXO1/3 (Phospho-S322+S325/pS318+S321) staining in human thyroid gland formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.