FoxO1/3 (phospho Ser322/S325) rabbit pAb

• Catalog Number: orb768176
• Category: Antibodies
• Description: Rabbit polyclonal antibody to FoxO1/3 (phospho-Ser322/S325)
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human FOXO1A/3A around the phosphorylation site of Ser322 and Ser325. AA range:291-340
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 97
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-FOXO1 antibody, anti-FKHR antibody, anti-FOXO
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of 3T3 cells using Phospho-FoxO1/3 (S322/S325) Polyclonal Antibody.

Western blot analysis of lysates from NIH/3T3 cells treated with Wortmannin 40nM 24h, using FOXO1A/3A (Phospho-Ser322 + Ser325) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).