FOXE1 Antibody

• Catalog Number: orb1247496
• Category: Antibodies
• Description: FOXE1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-AYPGGIDRFVSAM
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments gave an approx 70kDa band in Human Thymus and Thyroid Gland lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 38.1kDa according to NP_004464.2. The 70kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:In paraffin embedded Human Thyroid Gland shows nuclear staining in thyrocytes. Recommended concentration: 2-4ug/ml. Paraffin embedded Human Testis. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: FOXE1
• UniProt ID: O00358
• NCBI: NP_004464.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: FOXE1, TTF2, forkhead box E1 (thyroid transcriptio
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments gave an approx 70kDa band in Human Thymus and Thyroid Gland lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 38.1kDa according to NP_004464.2. The 70kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:In paraffin embedded Human Thyroid Gland shows nuclear staining in thyrocytes. Recommended concentration: 2-4ug/ml. Paraffin embedded Human Testis. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247496 (2 ug/ml) staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

orb1247496 (5 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with Tris/EDTA buffer pH 9, AP-staining.