FOXA1 Antibody

• Catalog Number: orb606445
• Category: Antibodies
• Description: Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate. [UniProt]
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: rFOXA1/1515
• Tested applications: FACS, IHC-P
• Reactivity: Human, Rat
• Isotype: Mouse IgG1, kappa
• Immunogen: A human recombinant partial protein corresponding to amin acids 372-472 was used as the immunogen for this recombinant FOXA1 antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Flow cytometry: 1-2ug/million cells
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant FOXA1 antibody is available for research use only.
• UniProt ID: P55317
• Storage: Store the recombinant FOXA1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant FOXA1 antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human prostate carcinoma with recombinant FOXA1 antibody (clone rFOXA1/1515). HIER: boil sections in 10mM Tris with 1mM EDTA, pH9 for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free recombinant FOXA1 antibody (clone rFOXA1/1515) as confirmation of integrity and purity.

FACS testing of PFA-fixed human MCF-7 cells with recombinant FOXA1 antibody (clone rFOXA1/1515); Red=isotype control, Blue=FOXA1 antibody.