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Catalog Number | orb312091 |
---|---|
Category | Antibodies |
Description | Fos B/FOSB Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IHC, WB |
Predicted Reactivity | Hamster |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Fos B (264-291aa LPFQTSQDAPPNLTASLFTHSEVQVLGD), different from the related mouse sequence by two amino acids. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 35928 MW |
UniProt ID | P53539 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Protein fosB;G0/G1 switch regulatory protein 3;FOS Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of SiHa cells using anti-FOSB antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of FOSB using anti-FOSB antibody.Lane 1:rat thymus tissue; 2:mouse thymus tissue; 3:mouse ovary tissue; 4:mouse spleen tissue; 5:mouse kidney tissue; 6:mouse RAW246.7 cell; 7:human HL-60 cell; 8:human THP-1 cell.
IHC analysis of FOSB using anti-FOSB antibody. FOSB was detected in paraffin-embedded section of human renal cancer tissue.
IHC analysis of FOSB using anti-FOSB antibody. FOSB was detected in paraffin-embedded section of human placenta tissue.
IHC analysis of FOSB using anti-FOSB antibody. FOSB was detected in paraffin-embedded section of mouse intestine tissue.
IHC analysis of FOSB using anti-FOSB antibody. FOSB was detected in paraffin-embedded section of rat intestine tissue.
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