Folate Hydrolase 1 Antibody / FOLH1 / PSMA

• Catalog Number: orb639630
• Category: Antibodies
• Description: Folate hydrolase 1 (FOLH1), also known as Prostate-specific membrane antigen (PSMA), is a type II transmembrane glycoprotein belonging to the M28 peptidase family. FOLH1 has two enzymatic activities, one as a prostate-specific integral membrane folate hydrolase and the other as a carboxypeptidase In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SPM500
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: A portion of amino acids 232-433 from the human protein was used as the immunogen for this Folate Hydrolase 1 antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Folate Hydrolase 1 antibody is available for research use only.
• UniProt ID: Q04609
• Storage: Store the Folate Hydrolase 1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human LNCaP cell lysate with Folate Hydrolase 1 antibody. Predicted molecular weight ~100 kDa.

IHC testing of FFPE human prostate cancer with Folate Hydrolase 1 antibody. Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Folate Hydrolase 1 antibody as confirmation of integrity and purity.