Folate Hydrolase 1 Antibody / FOLH1 / PSMA

• Catalog Number: orb639556
• Category: Antibodies
• Description: Folate hydrolase 1 (FOLH1), also known as Prostate-specific membrane antigen (PSMA), has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Involved in prostate tumor progression. [UniProt]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: GCP2-1
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: A portion of amino acids 232-433 from the human protein was used as the immunogen for this FOLH1 antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This FOLH1 antibody is available for research use only.
• UniProt ID: Q04609
• Storage: Store the FOLH1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human LNCaP cell lysate with FOLH1 antibody. Predicted molecular weight ~100 kDa.

IHC testing of FFPE human prostate carcinoma with FOLH1 antibody. Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.