FNIP1 Antibody: HRP

• Catalog Number: orb55607
• Category: Antibodies
• Description: Rabbit polyclonal antibody against FNIP1 conjugated to HRP.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide from the mid-protein of Human FNIP1
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000); ICC/IF (1:100); IHC (1:50)
• Conjugation: HRP
• MW: 130 kDa
• Target: FNIP1
• Entrez: 96459
• UniProt ID: Q8TF40
• NCBI: NP_001008738.2
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide and 0.23mM Citrate in dH2O
• Alternative names: FNIP1 antibody, Folliculin-interacting protein 1 a
• Note: For research use only
• Application notes: A 1:1000 dilution of SPC-718 was sufficient for detection of FNIP1 in 15 µg of mouse kidney cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-FNIP1 Polyclonal Antibody. Tissue: C2C12 Cells (Mouse Myoblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-FNIP1 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) FNIP1 Antibody (D) Composite.

Immunohistochemistry analysis using Rabbit Anti-FNP1 Polyclonal Antibody. Tissue: Renal Cell Carcinoma. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-FNP1 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.

Immunohistochemistry analysis using Rabbit Anti-FNP1 Polyclonal Antibody. Tissue: Renal Cell Carcinoma. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-FNP1 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.

Western blot analysis of Mouse, Rat Kidney showing detection of ~131 kDa FNIP1 protein using Rabbit Anti-FNIP1 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Kidney cell lysates. Lane 3: Rat Kidney cell lysates. Load: 20 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-FNIP1 Polyclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~131 kDa.