FN1 Antibody / Fibronectin

• Catalog Number: orb699486
• Category: Antibodies
• Description: Fibronectins are disulfide-linked, dimeric glycoproteins of ~440kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Epitope of this MAb is located in the 2nd-3rd type-III repeats of fibronectin. Fibronectins are extracellular matrix glycoproteins that are essential for embryonic development. Fibronectins are also involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. This MAb reacts with human cellular fibronectin, but not plasma fibronectin.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: FN1/2950
• Tested applications: ELISA, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A portion of amino acids 467-595 from the human protein was used as the immunogen for the Fibronectin antibody.
• Dilution range: ELISA (order Ab without BSA for coating),Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Fibronectin antibody is available for research use only.
• UniProt ID: P02751
• Storage: Store the Fibronectin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Fibronectin antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human lung with Fibronectin antibody (clone FN1/2950). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lung with Fibronectin antibody (clone FN1/2950). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Fibronectin antibody (clone FN1/2950). These results demonstrate the foremost specificity of the FN1/2950 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free Fibronectin antibody (clone FN1/2950) as confirmation of integrity and purity.