FLVCR1 Antibody

• Catalog Number: orb1247505
• Category: Antibodies
• Description: FLVCR1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-KTVMLSKQSESAI
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 55kDa band observed in Human Bone Marrow lysates (calculated MW of 59.9kDa according to NP_054772.1). Recommended concentration: 0.1-0.3ug/ml. An additional band of unknown identity was also consistently observed at 26kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Anonymous customer reported uccessfully use in WB on cell line K562.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: FLVCR1
• UniProt ID: Q9Y5Y0
• NCBI: NP_054772.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: FLVCR1, feline leukemia virus subgroup C cellular
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 55kDa band observed in Human Bone Marrow lysates (calculated MW of 59.9kDa according to NP_054772.1). Recommended concentration: 0.1-0.3ug/ml. An additional band of unknown identity was also consistently observed at 26kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Anonymous customer reported uccessfully use in WB on cell line K562.
• Expiration Date: 12 months from date of receipt.

orb1247505 (0.1 ug/ml) staining of Human Bone Marrow lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.