Fluorescein Conjugated Mouse IgG (H&L)

• Catalog Number: orb347422
• Category: Antibodies
• Description: Fluorescein Conjugated Mouse IgG (H&L) Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: DOT, FC, FLISA, IF, WB
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: Mouse IgG whole molecule
• Concentration: 1.0 mg/mL
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: Goat Anti-Mouse IgG [H&L] FITC Conjugated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgG and Mouse Serum.
• Conjugation: FITC
• Biological Activity: 3.5
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.1% (w/v) Sodium Azide
• Alternative names: Goat Anti-Mouse IgG Secondary Antibody fluorescein
• Note: For research use only
• Application notes: Anti-Mouse IgG FITC Conjugated Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, flow cytometry, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Expiration Date: 12 months from date of receipt.

Dot Blot Results of Goat Anti-Mouse IgG Fluorescein Conjugated Antibody. Mouse IgG (p/n orb2652749) sample loaded 1-100 ng, 2- 33.33 ng, 3- 11.11 ng, 4- 3.70 ng, 5- 1.23 ng. Goat Anti-Mouse IgG Fluorescein Conjugated Antibody at 1.0 µg/ml for 60 mins at RT. Blocking Buffer for Fluorescent Western Blotting (p/n orb348637) for 60 mins at RT. Chemi filter.

Western Blot of Goat anti-Mouse IgG Fluorescein Conjugated Secondary Antibody. Lane 1: Mouse IgG. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein goat secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): None.