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Fluorescein Conjugated F(ab')2 Fragment of Affinity Purified Mouse IgG (H&L) Pre-Adsorbed

Fluorescein Conjugated F(ab')2 Fragment of Affinity Purified Mouse IgG (H&L) Pre-Adsorbed

Catalog Number: orb348227

DispatchUsually dispatched within 5-10 working days
$ 330.00
Catalog Numberorb348227
CategoryAntibodies
DescriptionFluorescein Conjugated F(ab')2 Fragment of Affinity Purified Mouse IgG (H&L) Pre-Adsorbed Antibody
Species/HostGoat
ClonalityPolyclonal
Tested applicationsFC, FLISA, IF
ReactivityMouse
IsotypeIgG F(ab')2
ImmunogenMouse IgG whole molecule
Concentration1.0 mg/mL
Dilution rangeFLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
Form/AppearanceLyophilized
PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Human Serum Proteins.
ConjugationFITC
StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Buffer/Preservatives0.01% (w/v) Sodium Azide
Alternative namesF(ab')2 Anti-Mouse IgG Fluorescein Conjugated Anti
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NoteFor research use only
Application notesThis product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Expiration Date12 months from date of receipt.