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Catalog Number | orb348227 |
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Category | Antibodies |
Description | Fluorescein Conjugated F(ab')2 Fragment of Affinity Purified Mouse IgG (H&L) Pre-Adsorbed Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | FC, FLISA, IF |
Reactivity | Mouse |
Isotype | IgG F(ab')2 |
Immunogen | Mouse IgG whole molecule |
Concentration | 1.0 mg/mL |
Dilution range | FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000 |
Form/Appearance | Lyophilized |
Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Human Serum Proteins. |
Conjugation | FITC |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | F(ab')2 Anti-Mouse IgG Fluorescein Conjugated Anti Read more... |
Note | For research use only |
Application notes | This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. |
Expiration Date | 12 months from date of receipt. |