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| Catalog Number | orb764360 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to Flt-1 (phospho-Tyr1333) |
| Clonality | Polyclonal |
| Tested applications | ELISA, IHC-P, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human VEGFR1 around the phosphorylation site of Tyr1333. AA range:1289-1338 |
| Concentration | 1 mg/ml |
| Dilution range | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications. |
| Conjugation | Unconjugated |
| MW | 151 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Alternative names | Anti-FLT1 antibody, anti-FLT antibody, anti-FRT an Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Immunofluorescence analysis of A549. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000 (room temperature, 50min).3, Picture B: DAPI (blue) 10min.
Western Blot analysis of various cells using Phospho-Flt-1 (Y1333) Polyclonal Antibody.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using VEGFR1 (Phospho-Tyr1333) Antibody.
Immunohistochemistry analysis of paraffin-embedded human brain, using VEGFR1 (Phospho-Tyr1333) Antibody. The picture on the right is blocked with the phospho peptide.
