Flag Tag (7E1) Mouse mAb Antibody

• Catalog Number: orb1562815
• Category: Antibodies
• Description: Flag Tag (7E1) Mouse mAb Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 7E1-10D2-3H1
• Tested applications: ICC, IP, WB
• Reactivity: Other
• Isotype: IgG1
• Immunogen: Synthetic Peptide of Flag-Tag
• Concentration: 1.3 mg/ml
• Dilution range: WB: 1/1000-1/10000 IF: 1/100-1/500 IP: 1/20
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Alternative names: DYKDDDDK Tag; Flag; FLAG
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of Flag Tag (7E1) ) in 293 cells transfected with a Flagtag protein using Flag Tag (7E1) antibody (red), and DAPI (blue).

Immunoprecipitation analysis of Flag Tag (7E1) in 1) untransfected 293 cell lysates 2) transfected 293 cell lysates with Flagtag fusion protein 3) IP (transfected 293+ normal mouse IgG+Protein G agarose) 4) IP (transfected 293+anti Flag mA+Protein G agarose).

Western blot analysis of Flag Tag (7E1) in CHO-K1 cells (C) or CHO-K1 cells transfected with different FLAGfusion proteins (A, B), using FlagTag antibody.