FKBP8 antibody

• Catalog Number: orb344645
• Category: Antibodies
• Description: FKBP8 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human FKBP8 protein.
• Concentration: 2.3 mg/mL
• Dilution range: ELISA: 1:25,000, WB: 1:500-1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was protein A purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads. This antibody is specific for human FKBP8 protein. A BLAST analysis was used to suggest partial cross-reactivity with FKBP8 from mouse and rat sources based on ~93% homology with the immunizing sequence. Cross-reactivity with FKBP8 from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: Q14318
• NCBI: 52630440
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-FKBP8 Antibody, FKBP 8, FKBP-38, 38 kD
• Note: For research use only
• Application notes: This protein A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 64 kDa in size corresponding to FKBP8 by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western blot using Biorbyt's protein A purified anti-FKBP8 antibody shows detection of exogenous FKBP8 in 50 µg of HEK293T whole cell lysate (lane 1). The results of peptide competition are shown in lane 2 where no specific staining is noted after the antibody is first incubated for 1h with the immunizing peptide in 5% BLOTTO prior to reaction with the membrane. The membrane was probed with the primary antibody at a 1:1000 dilution in 5% BLOTTO at 4°C, overnight.