FKBP8 antibody

• Catalog Number: orb344644
• Category: Antibodies
• Description: FKBP8 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human FKBP8 protein.
• Concentration: 2.3 mg/mL
• Dilution range: ELISA: 1:25,000, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was protein A purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads. This antibody is specific for human FKBP8 protein. A BLAST analysis was used to suggest partial cross-reactivity with FKBP8 from mouse and rat sources based on ~93% homology with the immunizing sequence. Cross-reactivity with FKBP8 from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: Q14318
• NCBI: 52630440
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-FKBP8 Antibody, FKBP 8, FKBP-38, 38 kD
• Note: For research use only
• Application notes: This protein A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 64 kDa in size corresponding to FKBP8 by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western blot using Biorbyt's protein A purified anti-FKBP8 antibody shows detection of exogenous FKBP8 in 50 µg of HEK293T whole cell lysate (lane 1). The results of peptide competition are shown in lane 2 where no specific staining is noted after the antibody is first incubated for 1h with the immunizing peptide in 5% BLOTTO prior to reaction with the membrane. The membrane was probed with the primary antibody at a 1:1000 dilution in 5% BLOTTO at 4°C, overnight.