FIV matrix protein p15 antibody

• Catalog Number: orb345579
• Category: Antibodies
• Description: FIV matrix protein p15 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Virus
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids from an internal region of FIV Matrix Protein p15.
• Concentration: 1.22 mg/mL
• Dilution range: ELISA: 1:5,000 - 1:20,000, IHC: User Optimized, WB: 1:1,000 - 1:10,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This affinity purified antibody is directed against FIV Matrix Protein p15. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
• Conjugation: Unconjugated
• UniProt ID: P16087
• NCBI: 120815
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Feline immunodeficiency virus matrix p
• Note: For research use only
• Application notes: This affinity purified antibody has been tested for use in ELISA and western blotting and suitable for immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15 kDa in size corresponding to FIV Matrix Protein p15 by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western blot using Biorbyt's affinity purified anti-FIV Matrix Protein p15 to detect p15 in the culture supernatant of FIV-infected feline CrFK cells (lane 2, arrowhead). Lane 1 is an uninfected control. Virions were enriched by ultracentrifugation, lysed, resolved by electrophoresis, and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:10000 dilution.