Fibronectin 1/FN1 (C-term) Antibody

• Catalog Number: orb1294256
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Fibronectin (C-term)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rabbit
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the C-terminus region.
• Dilution range: Western Blot 1:500 Immunofluorescence 1:200–1:500 Immunohistochemistry (Paraffin) 1:100–1:200
• Conjugation: Unconjugated
• Target: Fibronectin 1 / FN1 (C-term)
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-Fibronectin antibody at 1/500 dilution. Lysates at 60 μg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294256 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded human hepatocellular carcinoma (HCC) labeling Fibronectin with orb1294256 at 1/100.

Immunohistochemical analysis of paraffin embedded human hepatocellular carcinoma (HCC) labeling Fibronectin with orb1294256 at 1/100.

Immunohistochemical analysis of paraffin embedded human breast carcinoma labeling Fibronectin with orb1294256 at 1/100.

Immunohistochemical analysis of paraffin embedded human breast carcinoma labeling Fibronectin with orb1294256 at 1/100.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Fibronectin with orb1294256 at 1/100.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294256 anti-Fibronectin antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Anti-Fibronectin antibody at 1/1000 dilution. Lysates at 50 μg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was incubated with orb1294256 overnight at 4°C.