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Catalog Number | orb1247472 |
---|---|
Category | Antibodies |
Description | FH Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | EIA, ELISA, IHC, WB |
Predicted Reactivity | Canine, Mouse, Rat |
Reactivity | Human |
Immunogen | The immunogen for this antibody is: C-HPNDHVNKSQSSND |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 48kDa band observed in Human Kidney, Liver and Lung lysates (calculated MW of 54.6kDa according to NP_000134.2). The observed molecular weight corresponds to earlier findings with antibodies from different sources. In transfected HEK293 transiently expressing Fumarase a band of approx. 55kDa is observed. This band is not observed in the non-transfected HEK293.Recommended concentration: 0.01-0.03ug/ml.Immunohistochemistry:Paraffin embedded Human Skeletal Muscle and Heart. Recommended concentration: 3.75ug/ml.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant Fumarase captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | FH |
UniProt ID | P07954 |
NCBI | NP_000134.2 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | fumarate hydratase, HLRCC, LRCC, MCL, MCUL1, fumar Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 48kDa band observed in Human Kidney, Liver and Lung lysates (calculated MW of 54.6kDa according to NP_000134.2). The observed molecular weight corresponds to earlier findings with antibodies from different sources. In transfected HEK293 transiently expressing Fumarase a band of approx. 55kDa is observed. This band is not observed in the non-transfected HEK293.Recommended concentration: 0.01-0.03ug/ml.Immunohistochemistry:Paraffin embedded Human Skeletal Muscle and Heart. Recommended concentration: 3.75ug/ml.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant Fumarase captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml |
Expiration Date | 12 months from date of receipt. |
orb1247472 (0.01 ug/ml) staining of Human Kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing Fumarase and probed with orb1247472 (mock transfection in first lane).
orb1247472 (1.5 ug/ml) as the reporter antibody.
orb1247472 (3.75 ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
orb1247472 (3.75 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
FC, ICC, IF, IHC, IHC-Fr, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, WB | |
Bovine, Canine, Equine, Guinea pig, Porcine, Rabbit, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC, WB | |
Human, Monkey, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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